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Fig. 3 | Genome Biology

Fig. 3

From: Functional analysis of androgen receptor mutations that confer anti-androgen resistance identified in circulating cell-free DNA from prostate cancer patients

Fig. 3

Steroid activation of AR mutants in comparison with the wild-type receptor in luciferase reporter assay. While most of the AR mutants showed similar or lower affinity to the activation by DHT (a), when compared to wild-type, several variants presented better activation by estradiol (b), progesterone (c), or hydrocortisone (d) than the wild-type. PC3 cells were transfected with both wild-type or mutated AR and a reporter plasmid pARR3-tk-luciferase. After 48 h post transfection, cells were treated with increasing concentrations of steroids. The graphs represent the average ± SEM of three independent experiments with four replicates each. The activity of each mutant in the presence of a steroid was normalized to the wild-type stimulated by 500 nM of the same steroid

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Genome Biology

ISSN: 1474-760X

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