Fig. 8
From: KIAA0556 is a novel ciliary basal body component mutated in Joubert syndrome
KIAA0556 is associated with several groups of ciliary proteins. a Visual representation of the preys identified in the TAP experiments. Proteins are clustered according to established protein complexes and the number of experiments in which each protein was identified. The full dataset is shown in Additional file 6. b Yeast two-hybrid (Y2H) experiment showing the interaction between fragments of the KIAA0556 protein (fused to the GAL4 activation domain; AD) and KATNBL1 and IFT88 (fused to the GAL4 DNA-binding domain; BD). An unrelated protein was used as negative control for KIAA0556 d1/2 LW; selective media lacking leucine, tryptophan. LWHA; selective media lacking leucine, tryptophan, histidine, adenine. The regions of KIAA0556 that interact with these proteins are depicted schematically in c. The predicted protein repeat domains are represented as d1 to d4. Additional file 7a shows all the KIAA0556 fragments tested in Y2H assays