Fig. 4

Genomic feature association of adipose-specific population differentially methylated regions (pDMRs), and transcription factor binding site (TFBS) motif analysis for adipose-specific low-methylated pDMRs. We determined pDMRs using a novel algorithm that weights the variance of CpGs methylation across individuals as well as the consistency of CpGs within the region (see “Methods”). a We associated the top 10 % of adipose-specific pDMRs with genomic features. The fold change of pDMR versus background (all CpGs detected in three or more individuals) is shown for each genomic feature. The red line demarks the border between enrichment (relative change > 1) and depletion (relative change < 1). b TFBS analysis was carried out for adipose-specific low-methylated pDMRs using the Homer software [33]. Shown are selected TFBS motifs including overall rank in the Homer analysis, p-value, forward consensus binding sequence, associated function, and references. The complete TFBS motif analysis result is available in Additional file 1: Table S6