Fig. 5

The splicing speed and associated features. The mRNA proportions at 1.5 min, 2.5 min, 5.0 min and steady state for 35 non-ribosomal protein (RP) intron-containing genes (a) and 82 RP intron-containing genes (b). The proportion of mRNA is estimated using the probabilistic model described in Additional file 1: Data and Methods RNA-seq data, and the area under the curve (AUC) score denotes the splicing speed as defined in the “Methods” section. Faster splicing transcripts cluster at the top, and slower splicing transcripts at the bottom. Red transcripts were also validated by reverse-transcription quantitative polymerase chain reaction (Fig. 6). All four colored bars are overlapping