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Fig. 1 | Genome Biology

Fig. 1

From: Transcriptome analysis in calorie-restricted rats implicates epigenetic and post-translational mechanisms in neuroprotection and aging

Fig. 1

Age-related differential gene expression in rats fed AL and subjected to CR. a Two simplified networks of related, statistically significant enriched Gene Ontology (GO) terms using the Cytoscape add-on ClueGO [30, 31]. The network comparing 6 months AL (6 m AL) and 28 months AL (28 m AL) is shown on the left and 12 months AL (12 m AL) and 28 months AL (28 m AL) is shown on the right. DE genes were used to generate a GO term network. The filled coloured circles (nodes) represent each statistically significant parent GO term. The lines (edges) between the nodes show that there are overlapping genes between terms. The Cytoscape add-on ClueGO allows enrichment analysis and the collapsing of GO terms into parent categories for each comparison. Each of the terms is statistically significant (Benjamini-Hochberg correction <0.05). Colours represent shared GO terms. The different sizes of the nodes relate to how many genes fall into the terms. b Heatmap of the DE genes with age across all AL and CR datasets (Table 2); created in R using the heatmap3 package. The y-axis represents all the DE genes. Red = up-regulated, blue = down-regulated, log2 fold change reported for statistically significant DE genes. White means no statistically significant change in expression. 6 m = 6 months of age, 12 m = 12 months of age, 28 m = 28 months of age

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