Skip to main content
Fig. 6 | Genome Biology

Fig. 6

From: Optimizing sgRNA structure to improve CRISPR-Cas9 knockout efficiency

Fig. 6

Testing the effect of modifications by lentiviral infection. TZM-bl cells (a) or JLTRG-R5 cells (b) were infected with Cas9-expressing lentivirus, and cells stably expressing Cas9 were selected. The indicated sgRNA (sp3 from Fig. 2a)-expressing cassettes were packaged into lentivirus and used to infect cells stably expressing Cas9 at MOI = 0.5. Knockout efficiency was determined as in Fig. 1b on the indicated days. The raw data are shown in Figure S12 in Additional file 1. O original, Mut mutant

Back to article page