Fig. 1
From: Quality control, modeling, and visualization of CRISPR screens with MAGeCK-VISPR
An overview of the MAGeCK-VISPR workflow. Given FASTQ files and an sgRNA design, the workflow employs several preprocessing steps, including using FastQC and MAGeCK to collect quality control metrics and calculate per-sgRNA read counts. Then, either MAGeCK-RRA or MAGeCK-MLE is used to determine essential genes under user-defined conditions. Finally, results are composed for visualization and interactive exploration in VISPR