Fig. 3

BAF regulates nucleosome positioning and recruits transcriptional machinery. a V-plot analysis of ATAC-seq fragments near p63 motif regions in both control (CTRLi) and BAF loss (BAFi) conditions. Schematic illustration of the V-plot analysis with phased nucleosome is included on the right. b Average diagram of nucleosome-free fragments (<100 bp) and mononucleosome fragment (180–247 bp) at p63 motif sites, demonstrating an average compaction of 40 bp at p63 sites with BAF loss. c UCSC genome browser tracks comparing ATAC-seq, H3K27Ac ChIP-seq, and Pol II ChIP-seq between CTRLi and BAFi, relative to p63 ChIP-seq at the HOPX TF locus. Decreased peaks with BAF loss are shaded with light blue. d–g Genome-wide average diagram of ChIP-seq (p63, H3K27Ac, Pol II, p300, and H3K27me3) comparing control to BAF loss. bp base pair, kb kilobase pair, TF transcription factor