Transcriptome-wide high-throughput deep m6A-seq reveals unique differential m6A methylation patterns between three organs in Arabidopsis thaliana

Table 2 Relationship between the m⁶A methlylation extent and the transcript level in three organs of the Arabidopsis m⁶A transcriptome

Replicates	Plant organs	High			Moderate			Low
Replicates	Plant organs	MFPKM	FPKM	Ratio	MFPKM	FPKM	Ratio	MFPKM	FPKM	Ratio
Replicate 1	Leaves	232.23	75.65	3.07	146.87	29.17	5.03	76.96	7.66	10.05
	Flowers	206.13	65.15	3.16	137.14	28.56	4.80	68.52	8.68	7.89
	Roots	156.63	62.28	2.51	111.23	28.44	3.91	56.22	7.35	7.65
Replicate 2	Leaves	221.14	88.55	2.50	176.68	17.94	9.84	99.06	3.75	26.42
	Flowers	152.77	80.11	1.91	120.33	15.29	7.87	69.34	3.49	19.87
	Roots	98.93	83.58	1.18	65.81	11.74	5.61	45.53	2.22	20.49

‘High’, ‘Moderate’, and ‘Low’ refer to three groupings of the transcript levels from the highest to the lowest FPKM in mRNA-seq. Each grouping included one-third numbers of the m⁶A modified transcripts. t-test on ratio of the average MFPKM in m⁶A-seq to the average FPKM in mRNA-seq in each grouping showed significantly different (P <0.005) ratios between three groupings

ISSN: 1474-760X