Fig. 8

Role of FGF19-induced phosphorylation of SHP at Thr-55 in functional interaction with SREBP-2 and inhibition of sterol biosynthetic genes. a Luciferase reporter assay: HepG2 cells were transfected with the plasmids indicated, treated with FGF19, and luciferase/β-galactosidase activities were measured. b Experimental outline for adenoviral experiments. c Mice were injected via the tail vein with control Ad-empty, Ad-SHP WT, or Ad-T55A and then 2 weeks later, livers were collected. For BA feeding (0.5 % CA diet) experiments, mice were fed normal diet (ND) or CA for 6 h and then, livers were collected. Liver extracts (pooled from n = 3) were prepared and protein levels of SHP, GFP, and actin were detected by IB. d Mice were injected via the tail vein with adenovirus and 1 week later, the injected mice were treated with vehicle or FGF19 for 2 h and CoIP assays were done in liver extracts and (e) ChIP assays were done using livers pooled from three mice. f Experimental outline. Mice were injected with Ad-SHP WT or the T55A mutant, and 2 weeks later, (g) mRNA levels of indicated genes and (h) liver and serum cholesterol levels were measured