Fig. 5

SHP was recruited to SREBP-2 target sterol biosynthesis-related genes and inhibits their expression. Mice were treated with vehicle or FGF19 and livers were pooled from three mice for ChIP assays (a, b) to examine occupancy of SHP (a) and SREBP-2 (b) at the indicated genes, and mRNA levels of genes near SHP binding peaks (c). Statistical significance was determined by the Student’s t-test (SEM, n = 3, *P <0.05, **P <0.01). d, e WT or SHP-KO mice were treated with vehicle or FGF19 for 6 h and livers were collected. d Effects of FGF19 on mRNA levels of indicated genes. e Hepatic protein levels of HMGCR in WT and SHP-KO mice were measured and relative HMGCR levels are shown (right)