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Table 1 Comparative summary of the three papers on CRISPR/Cas-induced resistance to geminivuruses in plants

From: Boosting plant immunity with CRISPR/Cas

  Ali et al. [1] Baltes et al. [8] Ji et al. [7]
Geminivirus species TYLCV, BCTV, MeMV BeYDV BSCTV
Geminivirus delivery Transient (Nb) Transient (Nb)/stable transgenics (Nb) Transient (Nb)/stable transgenics (Nb, At)
sgRNA expressed from: TRV T-DNA T-DNA
Cas9 expressed from: T-DNA T-DNA T-DNA
Geminivirus virulence assay Semi-qPCR, RCA, Southern blot GFP, CFUs (Escherichia coli) qPCR, Southern blot
Geminivirus virulence test in systemic tissue Yes Yes Yes
Geminivirus symptoms assayed Yes, in transient Yes, in stable transgenic lines Yes, in stable transgenic lines
  1. At Arabidopsis thaliana, BCTV beet curly top virus, BeYDV bean yellow dwarf virus, BSCTV beet severe curly top virus, CFUs, colony-forming units, GFP green fluorescent protein, MeMV Merremia mosaic virus, Nb Nicotiana benthamiana, qPCR quantitative PCR, RCA rolling-circle amplification, T-DNA transfer DNA, TRV tobacco rattle virus, TYLCV tomato yellow leaf curl virus