Table 1 Comparative summary of the three papers on CRISPR/Cas-induced resistance to geminivuruses in plants
| Â | Ali et al. [1] | Baltes et al. [8] | Ji et al. [7] |
|---|---|---|---|
Geminivirus species | TYLCV, BCTV, MeMV | BeYDV | BSCTV |
Geminivirus delivery | Transient (Nb) | Transient (Nb)/stable transgenics (Nb) | Transient (Nb)/stable transgenics (Nb, At) |
sgRNA expressed from: | TRV | T-DNA | T-DNA |
Cas9 expressed from: | T-DNA | T-DNA | T-DNA |
Geminivirus virulence assay | Semi-qPCR, RCA, Southern blot | GFP, CFUs (Escherichia coli) | qPCR, Southern blot |
Geminivirus virulence test in systemic tissue | Yes | Yes | Yes |
Geminivirus symptoms assayed | Yes, in transient | Yes, in stable transgenic lines | Yes, in stable transgenic lines |