Fig. 4
Glucocorticoid receptor activation regulates the expression of genes with transcription start sites (TSS) near epigenetic clock CpGs. Gene TSS near epigenetic clock CpGs were identified based on the annotation from [43]. The volcano plot shows DEX-induced fold change in gene expression plotted against their corrected P values (q values). The dotted red line represents the corrected level of statistical significance (q = 5 × 10−2) after FDR correction for multiple comparisons. Among the 216 unique array probes, 167 probes, corresponding to 139 unique genes, showed significant changes in gene expression following DEX. Fifty-eight per cent of these probes (n = 97) showed upregulation and 42 % (n = 70) showed downregulation. The mean (SD, range) distance of each regulated gene TSS to the corresponding epigenetic clock CpGs was ±419.3 bp (336.65 bp, range 1 to 1,423 bp). Marked in red are the probes showing fold changes in gene expression >1.1. Further details are provided in Additional file 2: Table S2