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Table 1 Summary of transgenerational RNA-guided Cas9 activity and segregation in barley

From: Induction of targeted, heritable mutations in barley and Brassica oleracea using RNA-guided Cas9 nuclease

T-DNA Construct T0 line T1 mutation type T1 line Number of T2 plants screened for T-DNA Plants without T-DNA Plants without T-DNA and with (homozygous/heterozygous) mutationsa
pPM19-1 T0-181 Homozygous T1-181_B5 4 0 -
    T1-181_E1 12 5 5/0
   Heterozygous T1-181_A11 12 3 1/1
    T1-181_B1 8 2 0/0
    T1-181_B8 11 0 -
    T1-181_C1 9 0 -
    T1-181_C12 1 0 -
    T1-181_C3 12 0 -
    T1-181_C4 12 1 0/0
    T1-181_C9 11 1 0/1
    T1-181_D11 12 4 1/1
    T1-181_D2 12 3 2/0
    T1-181_D9 2 0 -
    T1-181_E4 12 0 -
    T1-181_G4 11 3 0/1
    T1-181_G5 12 1 0/0
    T1-181_H2 12 3 1/1
    T1-181_H5 12 0 -
    T1-181_H9 12 4 0/0
  T0-122 Heterozygous T1-122_B11 12 4 2/1
    T1-122_C6 12 1 0/1
    T1-122_F12 12 4 0/3
    T1-122_H2 12 0 -
    T1-122_H4 12 3 0/0
    T1-122_H9 12 0 -
pPM19-3 T0-211 Heterozygous T1-211_B11 12 3 0/0
    T1-211_D10 12 0 -
    T1-211_G4 7 0 -
  1. aHyphens (-) indicate that all plants had presence of the T-DNA construct, and thus were not tested