Skip to main content
Fig. 7 | Genome Biology

Fig. 7

From: Induction of targeted, heritable mutations in barley and Brassica oleracea using RNA-guided Cas9 nuclease

Fig. 7

Frequency of on- and off-target Cas9 activity in L2F1_8.2 T1 Brassica plants. a The alignment of sgRNA1BolC.GA4.a and sgRNA2BolC.GA4.a target sequences in BolC.GA4.a with their corresponding sequences in BolC.GA4.b. Hyphens represent alignment matches while mismatches are shown in black highlight and white font. The PAM is highlighted in red and numbering of nucleotides is relative to the PAM. b Percentage of the T1 plants with mutations in BolC.GA4.a and BolC.GA4.b. Dark and light grey bars represent the percentages of BolC.GA4.a and BolC.GA4.b editing, respectively. N = 90 plants

Back to article page

Genome Biology

ISSN: 1474-760X

Contact us