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Fig. 6 | Genome Biology

Fig. 6

From: Induction of targeted, heritable mutations in barley and Brassica oleracea using RNA-guided Cas9 nuclease

Fig. 6

Mutations in BolC.GA4.a result in dwarf stature and affect the pod valve margin. a Wild-type B. oleracea DH1012 (left) and L2F1_A with a mutation in BolC.GA4.a showing a severe dwarf phenotype. Scale bar 10 cm. b Height of homozygous T1 plants with wild type (n = 11) or bolC.ga4.a mutant (n = 16) alleles. c Schematic cross section of B. oleracea pod with replum/valve margin region indicated by dashed square. Lignified tissue is indicated in red, unlignified cells are indicated in blue, and developing seeds are in green. d, e Cross-section of replum valve margin region of B. oleracea wild-type pod (d) and L2F1_A mutant pod (e); scale bars 200 μm

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