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Fig. 1 | Genome Biology

Fig. 1

From: Induction of targeted, heritable mutations in barley and Brassica oleracea using RNA-guided Cas9 nuclease

Fig. 1

Barley HvPM19 and B. oleracea BolC.GA4.a gene models and target sequences. a Morex HVVMRXALLmA0022M08_scaffold7 sequence contains the four barley HvPM19 gene copies (filled arrows). The target sequences for sgRNAHvPM19-1 and sgRNAHvPM19-3 (grey highlight) are shown below their respective gene models, with the protospacer-adjacent motif (PAM) highlighted in red. Recognition sequences for the restriction endonucleases SapI and MaeIII are underlined. b The B. oleracea BolC.GA4.a gene model includes two exons (filled boxes) separated by an intron (represented by a solid line). The B. oleracea BolC.GA4.a sequences for sgRNA1BolC.GA4.a (Target 1) and sgRNA2BolC.GA4.a (Target 2) are shown below the target regions in grey highlight with the PAM highlighted in red. Recognition sequences for the restriction endonucleases AflII, HaeIII and HphI are underlined. Primers for mutant detection are shown in both panels and detailed in Additional file 3

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