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Fig. 7 | Genome Biology

Fig. 7

From: P16-specific DNA methylation by engineered zinc finger methyltransferase inactivates gene transcription and promotes cancer metastasis

Fig. 7

Effect of varying P16 expression on migration. a Rescue assay demonstrating the effect of enforced P16 overexpression on migration of the P16-dnmt stably transfected BGC823 cells treated with doxycycline for 2 weeks. b Migration assay results after 24-h and 48-h transient siRNA transfection in GES-1 and BGC823 cell lines. P16 qRT-PCR results are also presented. c Migration capacity of A549 cells (lacking P16 alleles) following stable transfection of P16-dnmt. After treatment with 0.25 μg/mL doxycycline for 1 week, these cells (4.0 × 104) were seeded into each well and incubated for 28 h. Western blot analysis of P16-Dnmt and P16 expression is also presented. Migration assays were independently repeated in triplicate

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Genome Biology

ISSN: 1474-760X

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