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Fig. 2 | Genome Biology

Fig. 2

From: P16-specific DNA methylation by engineered zinc finger methyltransferase inactivates gene transcription and promotes cancer metastasis

Fig. 2

Comparison of DNA binding capacity, DNA methylation induction, and P16 expression levels between P16-dnmt and the R882H mutant in HEK293T and BGC823 cells 72 h following transient transfection. a Chromatin-immunoprecipitation (ChIP)-PCR results comparing P16 promoter DNA binding for P16-Dnmt and R882H protein; b Methylation-specific PCR (MSP) detecting methylated and unmethylated P16 alleles; c Quantitative RT-PCR indicating P16 mRNA levels; d Western blot of P16 and Myc/P16-Dnmt protein levels

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Genome Biology

ISSN: 1474-760X

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