Fig. 3
From: Rapid characterization of CRISPR-Cas9 protospacer adjacent motif sequence elements
Identification of Type II CRISPR-Cas elements in Brevibacillus laterosporus SSP360D4 CRISPR-Cas system. a An illustration of the genomic DNA region from the Type II CRISPR-Cas system from Brevibacillus laterosporus SSP360D4. b Comparison of Type II CRISPR array repeat sequences identified in Brevibacillus laterosporus SSP360D4. c The ‘direct’ and ‘reverse’ tracrRNA and CRISPR array transcriptional scenarios for the Type II CRISPR-Cas system from Brevibacillus laterosporus SSP360D4. d An agarose gel with reaction products, indicating that only the ‘direct’ sgRNA (dir sgRNA), but not the ‘reverse’ sgRNA (rev sgRNA) support plasmid library cleavage in combination with the Cas9 endonuclease originating from Brevibacillus laterosporus SSP360D4