Fig. 4

a Sequences of off-target sites identified by GUIDE-seq for SpCas9 and SaCas9 when directed by gRNA to VEGFA site 3. The intended target sequence is shown in the top line with the guide sequence on the left and the PAM on the right. On-target reads are shown in bold, and mismatches found in off-target sequences are highlighted in color. Off-target sites found in both datasets are matched with grey lines. Previously identified SpCas9 off-target sites are indicated with asterisks. b Histograms of mapped reads at off-target loci