Skip to main content
Fig. 3 | Genome Biology

Fig. 3

From: Characterization of Staphylococcus aureus Cas9: a smaller Cas9 for all-in-one adeno-associated virus delivery and paired nickase applications

Fig. 3

a. AAV constructs containing SaCas9 with a U6 promoter driving a VEGFA-15 gRNA, with a U6 promoter driving a CCR5-L2 gRNA, and with both U6-gRNAs. b Indel rates at VEGFA and CCR5 loci as measured by T7E1. Data are shown as means ± standard error of the mean (SEM) (N ≥ 3). N.D. none detected. c Western blots for Flag (SaCas9) and Tubulin confirming expression of SaCas9 in transduced cells. d AAV constructs containing D10A SaCas9 with different pairs of VEGFA-targeting gRNA expression cassettes. e Indel rates at VEGFA as measured by TOPO cloning and sequencing. Data are shown as means ± SEM (N = 2). N.D. none detected. f Western blots for Flag (SaCas9) and GAPDH confirming expression of D10A SaCas9 in transduced cells

Back to article page