Fig. 2From: Characterization of Staphylococcus aureus Cas9: a smaller Cas9 for all-in-one adeno-associated virus delivery and paired nickase applicationsD10A and N580A SaCas9 nickase data. a Sequence of the target locus (VEGFA) with five gRNAs annotated in grey. b Wild-type (WT) SaCas9, D10A and N580A SaCas9 nickases generating indels with single and dual gRNAs. Percentage indels on the y-axis represents on-target cleavage rates as measured by TOPO sequencing. Data are shown as means ± standard error of the mean (SEM) (N = 2). N.D. none detected. c Indel distribution of insertions and deletions from each SaCas9 type. Wild-type SaCas9 data shown are from transfections with single gRNAs, whereas D10A and N580A data shown are from transfections with dual gRNAs. d Indel rates as measured by TOPO sequencing for D10A SaCas9 and pairs of gRNAs with offset distances (between the 5′ ends of the gRNAs) ranging from -150 bp to 200 bpBack to article page