Fig. 3

Widespread functional reorganization of the rat medial prefrontal cortex transcriptome throughout the estrous cycle. a Enrichment map depicting the clusters of differentially modulated pathways between proestrus and diestrus females identified by the gene-set enrichment analysis. The area of each node, representing a gene set (functional pathway), corresponds to the number of genes of the gene set it contains, and the edge thickness is proportional to the number of genes overlapping between the two connected nodes. Pathways related to translation, degradation, oxidative phosphorylation, and transcription are associated with diestrus females, whereas extracellular matrix interactions, as well as insulin and synaptic signals transduction are associated with proestrus females. b treemap representation of Cellular Compartment terms from the Gene Ontology database, showing a marked enrichment of neuronal and synaptic genes. The size of each rectangle is proportional to the -log10 of the p-value (the bigger the rectangle, the more significant the enrichment). c The enrichment analysis of pathways from the Kyoto Encyclopedia of Genes and Genomes reveals a widespread alteration of signal transduction pathways, including neuronal and synaptic, as well as translation processes