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Fig. 1 | Genome Biology

Fig. 1

From: The estrous cycle surpasses sex differences in regulating the transcriptome in the rat medial prefrontal cortex and reveals an underlying role of early growth response 1

Fig. 1

A sexually biased transcriptome in the rat medial prefrontal cortex (mPFC) and effect of the estrous cycle. a A principal component analysis separates males and females along the second axis while proestrus and diestrus females are clustered separately along the first axis. b The hierarchical clustering of the 70 genes showing the most variance (rlog-transformed) revealed more similarity of diestrus females to males over proestrus females. c, e-g Representation of the log2 fold-change over the averaged normalized read counts, with significantly different genes at the false discovery rate (FDR) 5 % threshold highlighted in red. d The sexually dimorphic genes in the rat mPFC do not show a sex-chromosome bias, and are distributed among all chromosomes. To account for differences in number of genes per chromosome, data were normalized to the total number of genes detected in our study on each chromosome. h The Venn diagram representing the number of differentially expressed genes (FDR 5 %) in all pairwise comparisons depicts a relatively small overlap between genes affected by the estrous cycle within females, and those sexually biased in either stage of the cycle. The area of each circle is proportional to the number of genes it contains. In (ac, e-g), values from the R package DESeq2 were used

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