Table 1 Parameters used for the acquisition of FRAP data
From: A transient ischemic environment induces reversible compaction of chromatin
H2B-mCherry | H1.1-GFP | |
|---|---|---|
FRAP image acquisition | 256 × 256 pixels | 256 × 256 pixels |
Effective pixel size 96.5 nm | Effective pixel size 96.5 nm | |
Excitation: 6 % power of 561 nm | Excitation: 13 % power of 488 nm | |
Emission: 602–711 nm | Emission: 500–591 nm | |
Bleaching protocol | One 256 × 256 pixel scan in 2 × 2 μm, 80 % 561 nm laser | 100 ms point bleach, 80 % 488 nm laser |
Time/frame | 0.328 s | 1 s |
FRAP read-out | 2 × 2 μm square ROI | 1.5 μm in diameter circle ROI |