Fig. 6

OND depletes intracellular ATP levels, inhibits transcription, induces relocation of the cellular polyamine pool to the nucleus and reduces the staining density of histone H3 with antibody. a The intracellular concentration of ATP in untreated, OND-exposed and recovering cells was determined using a luciferase dependent assay. b Global rates of transcription, determined by the incorporation of bromouridine into RNA, in untreated cells, cells under OND and cells recovering from OND are presented. HL-1 cells, either untreated or subjected to 1 hour of OND, were fixed, permeabilized and stained either with anti-polyamine antibody (c, d) or with anti-total H3 antibody (e, f) and counterstained with the fluorescent DNA binding dye Hoechst 33342. Cells were then examined using confocal microscopy. The content of immunostained histone H3 was evaluated by SMLM in untreated (e) and OND-treated (f) HL-1 cells. BrU bromouridine. The error bars represent the standard deviation of three independent samples