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Fig. 2 | Genome Biology

Fig. 2

From: A transient ischemic environment induces reversible compaction of chromatin

Fig. 2

Alternative dyes and labeling methodologies confirm OND-induced compaction of chromatin. HL-1 cells, either untreated (ac) or exposed to 1 hour of OND (df) were fixed, permeabilized, stained with the DNA binding dye YOYO-1 and subjected to SMLM (a, b, d, e). Alternatively, cells were labeled for 24 hours with 10 μM 5-ethynyl-2′-deoxyuridine (EdU), and then were either untreated (gi) or subjected to 1 hour of OND (jl). Following fixation, EdU incorporated into DNA was coupled via click chemistry to AlexaFluor 488, as described [46], and nuclear DNA determined by SMLM (g, h, j, k). The dashed boxes in (a, d, g, j) are shown as zoomed views in (b), (e), (h) and (k), respectively. For comparison, wide-field images of the inset regions are shown in (c, f, i, l). Chromatin voids are indicated by an asterisk, with atolls marked by an arrow

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