Fig. 1

Oxygen and nutrient deprivation induces compaction of chromatin. HL-1 cells were fixed, permeabilized, and immunostained with anti-acetylated histone H3K14 and then counter-stained with Vybrant DyeCycle Violet. Two-color SMLM was performed on untreated HL-1 cells (a, b) or on cells exposed to 1 hour of OND (d, e). The dashed boxes in (a, d) are shown as zoomed views in (b) and (e), respectively. For comparison, wide-field images of the inset regions are shown in (c, f). Chromatin voids are indicated by asterisks and atolls marked by the arrow. Representative SMLM images of Vybrant Dyecycle Violet-stained nuclei, either untreated, subjected to 1 hour of OND or 5, 15, 60 and 240 minutes after release from OND are shown in (g). A discriminatory threshold (pixel intensity ≤ 50) was applied to the experimental set of SMLM imaged nuclei (a minimum of nine cells were imaged), with box plots and representative images describing the median and range of the proportion of the nucleus with chromatin shown in (h). P values compared with untreated are reported above the box plots. UT untreated