Fig. 2

Temporal expression of circRNAs in cortex. a Clustered heatmap showing expression patterns of the most highly expressed circRNAs (left) and corresponding linear host transcripts (right) in matching order. The Pearson correlation coefficient between circRNAs and linear hosts is 0.52. CircRNA clusters with similar expression patterns are numbered and described in the text. b CircRNA relative to host gene expression for the highest expressed circRNAs. This shows changes in circRNA expression level independent of the host gene expression. Grey tiles indicate ratios which were not calculated due to absent expression of one of the species. CircRNAs with undetected hosts are omitted. c, d Expression of circRNAs in embryonic cortex at E23 versus E115 (c) and E60 versus E80 (d). Diagonal lines indicate twofold up- and down-regulation. e, f Analysis of the impact of mRNA host gene expression change on circRNA expression change in embryonic cortex at E23 versus E115 (e) and E60 versus E80 (f). Diagonal lines indicate twofold up- and down-regulation. CircRNA/host expression changes that differ by less than twofold are shown in gray. For the other circRNAs (colored), expression levels change independently of host mRNAs