Table 1 Gene targeting frequencies in tomato cotelydons
From: High-frequency, precise modification of the tomato genome
T-DNA construct | Number of transformed cotyledons | Purple calli | Number of purple calli/transformed cotelydons (%) | Gene targeting frequency |
|---|---|---|---|---|
pTC147 (35S::ANT1 T-DNA) | 167 | 126 | 75.45 | NA |
pTC144 (BeYDV with TALENs 1193/1194) | 1881a | 137 | 7.28 | 9.65 × 10−2 |
pTC217 (BeYDV with Cas9/gRNA1b) | 216 | 19 | 8.80 | 11.66 × 10−2 |
pTC223 (BeYDV with Cas9/gRNA7) | 218 | 6 | 2.75 | 3.65 × 10−2 |
pTC150 (BeYDV with donor-only) | 200 | 0 | 0.00 | <0.66 × 10−2 |
pTC206 (BeYDV with TALENs 1193/1194)c | 183b | 0 | 0.00 | <0.72 × 10−2 |
pTC146 (BeYDV with donor, TALENs outside of the replicon) | 199 | 7 | 3.52 | 4.67 × 10−2 |
pTC151 (Conventional T-DNA) | 208 | 2 | 0.96 | 1.27 × 10−2 |
pTC208 (ToLCV with TALENs 1193/1194) | 273c | 8 | 2.93 | 3.88 × 10−2 |