Fig. 3
From: High-frequency, precise modification of the tomato genome
PCR analysis of targeted insertions in 16 purple calli obtained from one transformation experiment. a Diagram of the ANT1 locus after gene targeting. Numbered arrows represent primers used in the study. b At the left junction, 11 of 16 purple calli gave the correct PCR product; 16 of 16 purple calli gave the correct product at the right junction. Products were obtained in all reactions with the PCR controls. Numbers represent purple calli corresponding to independent GT events. M 2-Log DNA ladder (New England Biolabs), WT wild type plant, NT no template control