Fig. 4
From: The utility of transposon mutagenesis for cancer studies in the era of genome editing
Use of transposon-mediated insertional mutagenesis (TMIM) screening to identify mutations that co-operate with specific genetic lesions associated with different stages of colorectal cancer development. The top panels illustrate a model of colorectal cancer initiation and progression [101], along with genetic alterations associated with these stages. TMIM screens using mouse models carrying mutations in corresponding genes have revealed that Apc was the predominant gene inactivated in tumors from all sensitizing genotypes apart from Smad4 KO/+ cases, where inactivation of the remaining wild-type Smad4 gene is the most frequent insertional event