Fig. 2
From: TLE6 mutation causes the earliest known human embryonic lethality
Abrogation of TLE6 protein phosphorylation due to TLE6 missense mutation. a Western blot using phospho-tag acrylamide gels showing a shift of phosphorylated TLE6 and significant reduction of phosphorylated TLE6 in patient lymphocytes compared to normal controls. GAPDH is shown as loading control. b Quantification of A. c Phosphorylation of TLE6 is reduced in control lymphocytes treated with 40 μM calf intestine phosphatase (CIP) and PKA inhibitor (PKI). d Quantification of C from two biological repeats and two technical repeats. e In vitro phosphorylation of wild-type and S510Y TLE6 by PKA in the presence of [γ32P] followed by autoradiography and immunoblotting using anti-TLE6. f Quantification of E from three independent repeats. Ctr: Control cells, P: Patient cells