Fig. 2
From: WU-CRISPR: characteristics of functional guide RNAs for the CRISPR/Cas9 system
Thermodynamic properties of the guide sequence (gRNA). Functional and non-functional gRNAs were compared in the analysis. a Structural stability of the gRNA as evaluated by self-folding free energy (ΔG). b Structural stability of the gRNA/target sequence duplex as evaluated by free energy calculation