Fig. 1
From: WU-CRISPR: characteristics of functional guide RNAs for the CRISPR/Cas9 system
Structural characteristics of sgRNAs. a Secondary structure of the sgRNA. The 20-nucleotide guide sequence is complementary to the target sequence and resides at the 5′ end of the sgRNA. The highlighted nucleotides could potentially base pair, leading to an extended stem-loop structure. b Statistical significance of position-specific nucleotide accessibility of functional sgRNAs compared with non-functional sgRNAs. c Comparison of position-specific nucleotide accessibilities between functional and non-functional sgRNAs