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Fig. 1 | Genome Biology

Fig. 1

From: gespeR: a statistical model for deconvoluting off-target-confounded RNA interference screens

Fig. 1

Gene-specific phenotypes (GSPs; red) estimated from off-target-confounded RNAi screens. a Schematic representation of a knockdown screen. RNAi reagents (e.g., siRNAs) target their intended on-target (black solid arrow) and additional off-target (grey dashed line arrows) genes. Each gene has a hidden GSP, whereas the observed reagent-specific phenotypes (RSPs; violet) correspond to the combined effect of on- and off-target genes. b Unlike RSPs, deconvoluted GSPs are expected to exhibit high concordance between distinct libraries containing different reagents targeting the same genes

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