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Table 3 HPLC analysis of tRNAPhe and tRNAVal(AAC) nucleoside content from human LCLs

From: Mutation in WDR4 impairs tRNA m7G46 methylation and causes a distinct form of microcephalic primordial dwarfism

Mod.

Mol. exp.

WDR4/WDR4

WDR4/wdr4-R170L

wdr4-R170L/wdr4-R170L

tRNAPhe

m7G

1

0.49 ± 0.10

0.46 ± 0.14

0.08 ± 0.02

Ψ

4

3.52 ± 0.06

3.82 ± 0.15

3.53 ± 0.15

Cm

1

0.71 ± 0.10

0.73 ± 0.02

0.86 ± 0.03

Gm

1

0.71 ± 0.07

0.63 ± 0.20

0.70 ± 0.17

m5C

1

0.61 ± 0.06

0.63 ± 0.04

0.88 ± 0.04

m2G

1

0.78 ± 0.05

0.66 ± 0.02

0.68 ± 0.05

tRNAVal(AAC)

m7G

1

0.52 ± 0.08

0.46 ± 0.02

0.35 ± 0.06

Ψ

3

3.10 ± 0.08

3.07 ± 0.11

2.94 ± 0.16

I

1

0.40 ± 0.03

0.39 ± 0.01

0.33 ± 0.04

m5C

2

1.66 ± 0.09

1.70 ± 0.09

1.58 ± 0.20

m2G

1

0.90 ± 0.09

0.87 ± 0.11

0.76 ± 0.01

  1. Mean and standard deviation based on three individual growths and RNA preparations