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Table 3 HPLC analysis of tRNAPhe and tRNAVal(AAC) nucleoside content from human LCLs

From: Mutation in WDR4 impairs tRNA m7G46 methylation and causes a distinct form of microcephalic primordial dwarfism

Mod. Mol. exp. WDR4/WDR4 WDR4/wdr4-R170L wdr4-R170L/wdr4-R170L
tRNAPhe
m7G 1 0.49 ± 0.10 0.46 ± 0.14 0.08 ± 0.02
Ψ 4 3.52 ± 0.06 3.82 ± 0.15 3.53 ± 0.15
Cm 1 0.71 ± 0.10 0.73 ± 0.02 0.86 ± 0.03
Gm 1 0.71 ± 0.07 0.63 ± 0.20 0.70 ± 0.17
m5C 1 0.61 ± 0.06 0.63 ± 0.04 0.88 ± 0.04
m2G 1 0.78 ± 0.05 0.66 ± 0.02 0.68 ± 0.05
tRNAVal(AAC)
m7G 1 0.52 ± 0.08 0.46 ± 0.02 0.35 ± 0.06
Ψ 3 3.10 ± 0.08 3.07 ± 0.11 2.94 ± 0.16
I 1 0.40 ± 0.03 0.39 ± 0.01 0.33 ± 0.04
m5C 2 1.66 ± 0.09 1.70 ± 0.09 1.58 ± 0.20
m2G 1 0.90 ± 0.09 0.87 ± 0.11 0.76 ± 0.01
  1. Mean and standard deviation based on three individual growths and RNA preparations