|
Mod.
|
Mol. exp.
|
wt (CEN vec)
|
trm82∆ (CEN TRM82)
|
trm82∆ (CEN vec)
|
trm82∆ (CEN trm82-K223L)
|
trm82∆ (CEN trm82-K223R)
|
|---|
|
30 °C
|
|
m7G
|
1
|
0.50 ± 0.07
|
0.53 ± 0.03
|
<0.03
|
0.45 ± 0.05
|
0.46 ± 0.07
|
|
Ψ
|
4
|
3.66 ± 0.10
|
3.87 ± 0.18
|
3.94 ± 0.18
|
3.89 ± 0.15
|
3.64 ± 0.08
|
|
I
|
1
|
0.76 ± 0.02
|
0.85 ± 0.07
|
0.91 ± 0.06
|
0.90 ± 0.05
|
0.77 ± 0.01
|
|
m5C
|
1
|
0.87 ± 0.03
|
0.94 ± 0.03
|
1.14 ± 0.05
|
0.97 ± 0.05
|
0.91 ± 0.03
|
|
m1G
|
1
|
0.81 ± 0.05
|
0.98 ± 0.05
|
0.99 ± 0.05
|
1.00 ± 0.08
|
0.83 ± 0.05
|
|
m2G
|
0
|
0.03 ± 0.01
|
0.05 ± 0.01
|
0.54 ± 0.10
|
0.07 ± 0.02
|
0.05 ± 0.01
|
|
37 °C
|
|
m7G
|
1
|
0.61 ± 0.04
|
0.50 ± 0.07
|
<0.03
|
0.25 ± 0.03
|
0.55 ± 0.11
|
|
Ψ
|
4
|
3.88 ± 0.10
|
3.93 ± 0.05
|
3.85 ± 0.15
|
3.99 ± 0.20
|
4.10 ± 0.08
|
|
I
|
1
|
0.80 ± 0.01
|
0.77 ± 0.02
|
0.74 ± 0.06
|
0.78 ± 0.07
|
0.85 ± 0.04
|
|
m5C
|
1
|
1.04 ± 0.04
|
1.19 ± 0.04
|
1.53 ± 0.08
|
1.42 ± 0.09
|
1.16 ± 0.07
|
|
m1G
|
1
|
0.87 ± 0.01
|
0.79 ± 0.03
|
0.79 ± 0.10
|
0.87 ± 0.06
|
0.94 ± 0.01
|
|
m2G
|
0
|
0.05 ± 0.03
|
0.10 ± 0.03
|
0.45 ± 0.03
|
0.27 ± 0.05
|
0.06 ± 0.01
|
- Mean and standard deviation based on three individual growths and RNA preparations
