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Table 2 HPLC analysis of tRNAVal(AAC) nucleoside content from an S. cerevisiae trm82∆ strain expressing TRM82 variants

From: Mutation in WDR4 impairs tRNA m7G46 methylation and causes a distinct form of microcephalic primordial dwarfism

Mod. Mol. exp. wt (CEN vec) trm82∆ (CEN TRM82) trm82∆ (CEN vec) trm82∆ (CEN trm82-K223L) trm82∆ (CEN trm82-K223R)
30 °C
m7G 1 0.50 ± 0.07 0.53 ± 0.03 <0.03 0.45 ± 0.05 0.46 ± 0.07
Ψ 4 3.66 ± 0.10 3.87 ± 0.18 3.94 ± 0.18 3.89 ± 0.15 3.64 ± 0.08
I 1 0.76 ± 0.02 0.85 ± 0.07 0.91 ± 0.06 0.90 ± 0.05 0.77 ± 0.01
m5C 1 0.87 ± 0.03 0.94 ± 0.03 1.14 ± 0.05 0.97 ± 0.05 0.91 ± 0.03
m1G 1 0.81 ± 0.05 0.98 ± 0.05 0.99 ± 0.05 1.00 ± 0.08 0.83 ± 0.05
m2G 0 0.03 ± 0.01 0.05 ± 0.01 0.54 ± 0.10 0.07 ± 0.02 0.05 ± 0.01
37 °C
m7G 1 0.61 ± 0.04 0.50 ± 0.07 <0.03 0.25 ± 0.03 0.55 ± 0.11
Ψ 4 3.88 ± 0.10 3.93 ± 0.05 3.85 ± 0.15 3.99 ± 0.20 4.10 ± 0.08
I 1 0.80 ± 0.01 0.77 ± 0.02 0.74 ± 0.06 0.78 ± 0.07 0.85 ± 0.04
m5C 1 1.04 ± 0.04 1.19 ± 0.04 1.53 ± 0.08 1.42 ± 0.09 1.16 ± 0.07
m1G 1 0.87 ± 0.01 0.79 ± 0.03 0.79 ± 0.10 0.87 ± 0.06 0.94 ± 0.01
m2G 0 0.05 ± 0.03 0.10 ± 0.03 0.45 ± 0.03 0.27 ± 0.05 0.06 ± 0.01
  1. Mean and standard deviation based on three individual growths and RNA preparations