Mod. | Mol. exp. | wt (CEN vec) | trm82∆ (CEN TRM82) | trm82∆ (CEN vec) | trm82∆ (CEN trm82-K223L) | trm82∆ (CEN trm82-K223R) |
---|---|---|---|---|---|---|
30 °C | ||||||
m7G | 1 | 0.41 ± 0.04 | 0.34 ± 0.03 | <0.03 | 0.21 ± 0.01 | 0.32 ± 0.04 |
Ψ | 2 | 2.09 ± 0.06 | 1.94 ± 0.03 | 1.85 ± 0.04 | 1.99 ± 0.07 | 2.06 ± 0.05 |
Cm | 1 | 0.89 ± 0.09 | 0.92 ± 0.05 | 0.93 ± 0.08 | 0.95 ± 0.03 | 0.93 ± 0.06 |
Gm | 1 | 0.72 ± 0.01 | 0.76 ± 0.07 | 0.76 ± 0.03 | 0.73 ± 0.08 | 0.75 ± 0.02 |
m5C | 2 | 1.53 ± 0.06 | 1.64 ± 0.07 | 1.69 ± 0.03 | 1.66 ± 0.06 | 1.59 ± 0.03 |
m2G | 1 | 0.78 ± 0.02 | 0.83 ± 0.04 | 0.87 ± 0.02 | 0.83 ± 0.04 | 0.82 ± 0.03 |
37 °C | ||||||
m7G | 1 | 0.37 ± 0.03 | 0.21 ± 0.03 | <0.03 | <0.03 | 0.18 ± 0.02 |
Ψ | 2 | 2.00 ± 0.08 | 1.91 ± 0.06 | 1.87 ± 0.01 | 1.82 ± 0.10 | 1.92 ± 0.01 |
Cm | 1 | 0.98 ± 0.08 | 1.00 ± 0.14 | 0.91 ± 0.02 | 0.93 ± 0.05 | 0.90 ± 0.04 |
Gm | 1 | 0.77 ± 0.03 | 0.84 ± 0.02 | 0.91 ± 0.05 | 0.96 ± 0.02 | 0.84 ± 0.02 |
m5C | 2 | 1.63 ± 0.03 | 1.69 ± 0.02 | 1.79 ± 0.05 | 1.76 ± 0.04 | 1.71 ± 0.02 |
m2G | 1 | 0.82 ± 0.05 | 0.88 ± 0.02 | 0.90 ± 0.07 | 0.94 ± 0.06 | 0.86 ± 0.02 |