Mod.
|
Mol. exp.
|
wt (CEN vec)
|
trm82∆ (CEN TRM82)
|
trm82∆ (CEN vec)
|
trm82∆ (CEN trm82-K223L)
|
trm82∆ (CEN trm82-K223R)
|
---|
30 °C
|
m7G
|
1
|
0.41 ± 0.04
|
0.34 ± 0.03
|
<0.03
|
0.21 ± 0.01
|
0.32 ± 0.04
|
Ψ
|
2
|
2.09 ± 0.06
|
1.94 ± 0.03
|
1.85 ± 0.04
|
1.99 ± 0.07
|
2.06 ± 0.05
|
Cm
|
1
|
0.89 ± 0.09
|
0.92 ± 0.05
|
0.93 ± 0.08
|
0.95 ± 0.03
|
0.93 ± 0.06
|
Gm
|
1
|
0.72 ± 0.01
|
0.76 ± 0.07
|
0.76 ± 0.03
|
0.73 ± 0.08
|
0.75 ± 0.02
|
m5C
|
2
|
1.53 ± 0.06
|
1.64 ± 0.07
|
1.69 ± 0.03
|
1.66 ± 0.06
|
1.59 ± 0.03
|
m2G
|
1
|
0.78 ± 0.02
|
0.83 ± 0.04
|
0.87 ± 0.02
|
0.83 ± 0.04
|
0.82 ± 0.03
|
37 °C
|
m7G
|
1
|
0.37 ± 0.03
|
0.21 ± 0.03
|
<0.03
|
<0.03
|
0.18 ± 0.02
|
Ψ
|
2
|
2.00 ± 0.08
|
1.91 ± 0.06
|
1.87 ± 0.01
|
1.82 ± 0.10
|
1.92 ± 0.01
|
Cm
|
1
|
0.98 ± 0.08
|
1.00 ± 0.14
|
0.91 ± 0.02
|
0.93 ± 0.05
|
0.90 ± 0.04
|
Gm
|
1
|
0.77 ± 0.03
|
0.84 ± 0.02
|
0.91 ± 0.05
|
0.96 ± 0.02
|
0.84 ± 0.02
|
m5C
|
2
|
1.63 ± 0.03
|
1.69 ± 0.02
|
1.79 ± 0.05
|
1.76 ± 0.04
|
1.71 ± 0.02
|
m2G
|
1
|
0.82 ± 0.05
|
0.88 ± 0.02
|
0.90 ± 0.07
|
0.94 ± 0.06
|
0.86 ± 0.02
|
- Mean and standard deviation based on three individual growths and RNA preparations