Skip to main content

Table 1 HPLC analysis of tRNAPhe nucleoside content from an S. cerevisiae trm82∆ strain expressing TRM82 variants

From: Mutation in WDR4 impairs tRNA m7G46 methylation and causes a distinct form of microcephalic primordial dwarfism

Mod. Mol. exp. wt (CEN vec) trm82∆ (CEN TRM82) trm82∆ (CEN vec) trm82∆ (CEN trm82-K223L) trm82∆ (CEN trm82-K223R)
30 °C
m7G 1 0.41 ± 0.04 0.34 ± 0.03 <0.03 0.21 ± 0.01 0.32 ± 0.04
Ψ 2 2.09 ± 0.06 1.94 ± 0.03 1.85 ± 0.04 1.99 ± 0.07 2.06 ± 0.05
Cm 1 0.89 ± 0.09 0.92 ± 0.05 0.93 ± 0.08 0.95 ± 0.03 0.93 ± 0.06
Gm 1 0.72 ± 0.01 0.76 ± 0.07 0.76 ± 0.03 0.73 ± 0.08 0.75 ± 0.02
m5C 2 1.53 ± 0.06 1.64 ± 0.07 1.69 ± 0.03 1.66 ± 0.06 1.59 ± 0.03
m2G 1 0.78 ± 0.02 0.83 ± 0.04 0.87 ± 0.02 0.83 ± 0.04 0.82 ± 0.03
37 °C
m7G 1 0.37 ± 0.03 0.21 ± 0.03 <0.03 <0.03 0.18 ± 0.02
Ψ 2 2.00 ± 0.08 1.91 ± 0.06 1.87 ± 0.01 1.82 ± 0.10 1.92 ± 0.01
Cm 1 0.98 ± 0.08 1.00 ± 0.14 0.91 ± 0.02 0.93 ± 0.05 0.90 ± 0.04
Gm 1 0.77 ± 0.03 0.84 ± 0.02 0.91 ± 0.05 0.96 ± 0.02 0.84 ± 0.02
m5C 2 1.63 ± 0.03 1.69 ± 0.02 1.79 ± 0.05 1.76 ± 0.04 1.71 ± 0.02
m2G 1 0.82 ± 0.05 0.88 ± 0.02 0.90 ± 0.07 0.94 ± 0.06 0.86 ± 0.02
  1. Mean and standard deviation based on three individual growths and RNA preparations