Fig. 4From: Determining exon connectivity in complex mRNAs by nanopore sequencingMinION sequencing of Dscam1 identified 7,874 isoforms. a Histogram of read length distribution for Drosophila head samples. b The total number of Dscam1 isoforms identified from MinION sequencing. c Cumulative distribution of Dscam1 isoforms with respect to expression. d Violin plot of the number of isoforms identified using 100 random pools of the indicated number of reads. e Plot of the estimated number of total isoforms present in the library using the capture-recapture method with two random pools of the indicated number of reads. The shaded blue area indicates the 95 % confidence interval. f Deconvoluted expression of Dscam1 exon cluster variants (top) and the isoform connectivity of two highly expressed Dscam1 isoforms (bottom)Back to article page