Fig. 6

Immunofluorescence shows features enriched at site of XIST RNA. The XIST RNA is identified by RNA FISH (green, except for H2AK119u1 (uH2A) photos, shown in greyscale) after 5 days DOX induction for the Xq integration clone. Co-IF with the antibodies listed was performed (shown in grayscale), with the merged image in color. Bars to the right show the proportion of XIST-positive HT1080 cells (integration into Xq) displaying enrichment of the indicated chromatin modification/protein