Fig. 6

Eleven percent of the transcripts deregulated in PfAlba1-Ty1 trophozoites directly bind to PfAlba1, with binding resulting in the translational repression of select components of the erythrocyte invasion machinery. a Venn diagrams were used to represent the overlap of transcripts identified in the co-IPed (co-IP; Additional file 2), in vitro-bound (In Vitro; Additional file 4), and differentially regulated (Exp.; Additional file 6) datasets. b Pie chart showing the functionally over-represented categories in the 105 PfAlba1-bound and deregulated transcripts. c Bootstrap hierarchical clustering was used to partition 14 transcripts upregulated in PfAlba1-Ty1 trophozoites based on their binding (log₂(fold change); blue shading) to PfAlba1-Ty1 (co-IP) and GST-PfAlba1 (In Vitro). The numbers in the boxes are the bootstrap scores. The resulting clusters were PfAlba1-bound (upper cluster) and PfAlba1-unbound (lower cluster). d Top panel: qRT-PCR analysis of the indicated genes was performed using mRNA isolated from empty vector or PfAlba1-Ty1 trophozoites. The y-axis denotes -ΔΔCt, calculated as in “Materials and methods”. Data represent the means of a minimum of three independent experiments ± standard error (error bars). Bottom panel: protein lysates prepared from empty vector or PfAlba1-Ty1 trophozoites were separated by denaturing gel electrophoresis and analyzed by western blotting with the indicated antibodies. Lysates prepared from either 3D7 rings (R ctrl) or schizonts (S ctrl) served as a control for protein detection, except for PfFIKK7.1. ** = Exceptions to the observed correlation between PfAlba1-RNA binding and translational repression