Skip to main content
Fig. 2 | Genome Biology

Fig. 2

From: The PfAlba1 RNA-binding protein is an important regulator of translational timing in Plasmodium falciparum blood stages

Fig. 2

PfAlba1 overexpression reduces intra-erythrocytic growth of P. falciparum. a Protein lysates prepared from empty vector or PfAlba1-Ty1 transfectants grown in the presence of increasing concentrations of blasticidin-S (BS; 2.5–20 μg/ml) were separated by denaturing gel electrophoresis and analyzed by western blotting with mouse anti-Ty1 antibodies, anti-PfAlba1 antibodies, or anti-PfAlba4 antibodies. PfAldolase served as a loading control. b The levels of PfAlba1-Ty1, as detected by the anti-Ty1 antibodies in (a), were measured by densitometry. For each BS concentration, after normalizing the PfAlba1-Ty1 signal to the PfAldolase signal, data were normalized to the 2.5 μg/ml sample. Data represent the means of a minimum of three independent experiments ± standard error (S.E.; error bars). c The growth of empty vector (left panel) or PfAlba1-Ty1 (right panel) parasites was measured by flow cytometry for 5 days in the presence of the indicated concentrations of BS (in μg/ml). The y-axis denotes the percentage parasitemia at each time point. Data represent the means of a minimum of three independent experiments ± S.E. (error bars). d The ~48 h IDC of empty vector or PfAlba1-Ty1 transfectants was monitored by flow cytometry. The y-axis denotes the percentage of ring stages at each time point. The vertical dashed line represents one replication cycle. Data represent the means of three independent experiments ± S.E. (error bars)

Back to article page