Fig. 5

H₂O₂ signal during cell growth primes cells to sustain long-term viability and to form hyperclusters upon starvation. a Top: summary scheme of Ctt1 catalase activity. Center: Rap1-GFP representative images of SP wild-type (WT) and ctt1∆ cultures. Quantification of the distribution of intensity and number of foci of Rap1-GFP images was performed with our in-house software. Pie charts at the bottom represent the percentage of cells with telomere hyperclusters (black) within the population. b Top: summary scheme of Sod2 superoxidase activity. Center: Rap1-GFP representative images of WT and GPD-SOD2 cultures at 4 days in YPD (late respiration). Quantification of the distribution of intensity and number of foci of Rap1-GFP images was performed as in (a). c The effect of H₂O₂ (1 mM) on hyperclustering commitment. WT yAT1684 cells undergoing fermentation with or without H₂O₂ treatment were starved for 16 h in water and then imaged. Representative fluorescent Rap1-GFP pictures are shown. Pie charts represent the percentage of cells with telomere hyperclusters (black) within the population. For each condition, more than 1000 cells were analyzed. Statistical tests were carried out using a two-proportion Z test. d Colony forming ability measured as percentage of colony forming units (CFU) of the cultures from (c) after 7 days of starvation. Cultures were plated at day 1 and day 7 of starvation and the ratio day 7/day 1 is reported. Standard deviations from three experiments are indicated