Fig. 1

Assemblathon 1 metassembly accuracy. Assembly contiguity and accuracy metrics are plotted at each merging step for all possible permutations of the five input assemblies: scaffold N50 (a), corrected contig N50 (b), duplicated reference bases (c), deleted reference bases (d), translocations (e), and relocations (f). For all plots, the x-axis represents the number of input assemblies being metassembled, with 1 being the starting assembly. The two horizontal red lines mark the final maximum and minimum value of the metric across all permutations. Most of the permutations are plotted in gray, while permutations of particular note are plotted with different colors: the pink line represents the permutation that has the maximum value in the final metassembly while the dark blue line represents the permutation with the minimum value. Also, the green line represents the permutation resulting from ordering the input assemblies by the overall rank reported in the Assemblathon 1 paper (Broad-BGI-WTSI-DOEJGI-CSHL), the light blue line represents the permutation obtained by ordering the input assemblies by scaffold N50 size (DOEJGI-Broad-WTSI-CSHL-BGI) while the brown line represents the order by contig N50 size (BGI-Broad-CSHL-WTSI-DOEJGI). Comp Ref Bases compressed reference bases, Dup Ref Bases duplicated reference bases