Fig. 6

The germ-free condition affects developmentally programmed 3′ CGI methylation and transcription in intestinal epithelial cells. a DNA methylation status at multiple CpG sites of five 3′ CGI-associated genes are compared between two conditions: CNV (N = 5, red) and GF (N = 5, green). The error bars represent standard error of the mean. The error bars for the GF group are not showing as they are smaller than the symbols. b Gene expression analysis of corresponding 3′ CGI genes. *P < 0.05, **P < 0.01 and ***P < 0.001, N.S. not significant