Fig. 5

The germ-free condition affects developmentally programmed CGI methylation. a Unsupervised hierarchical clustering analysis on the basis of DNA methylation profiling in mouse colon under conventional (CNV) conditions. Each row indicates a CpG site and the corresponding gene name is indicated on the right. Each column indicates a sample analyzed and the corresponding age is indicated on the bottom. Methylation levels range from unmethylated (blue) to fully methylated (red), as indicated by the color legend at the bottom of the graphs. b DNA methylation profiling in mouse colon under germ-free (GF) conditions. The orders for CpG sites and age groups are pre-defined based on the conventional samples. Purple boxes highlight 3′ CGI-associated CpGs at which the methylation levels are significantly altered by germ-free conditions