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Fig. 5 | Genome Biology

Fig. 5

From: Heterochromatin Protein 1β (HP1β) has distinct functions and distinct nuclear distribution in pluripotent versus differentiated cells

Fig. 5

HP1β is highly expressed and diffuse in nuclei of pluripotent cells. a Confocal images of MEFs (top), R1 ESCs (middle) and Rr5 iPSCs (bottom) immunostained for Nanog (green, middle), HP1β (red, right) and counterstained with DAPI (blue, left). Asterisks indicate examples of MEFs used as a feeder layer in the culture of the pluripotent cells. b Quantification of the fluorescence intensities of Nanog (green bars) and HP1β (red bars) for the three cell types (n ≥ 26). Nanog is used as a marker for pluripotent cells; the fluorescence intensity of the background intensity was subtracted. c Number of HP1β foci in the different cell types. Error bars in (b) and (c) represent standard error of the mean. d Confocal images of R1 ESCs immunostained for Nanog (green, middle), HP1γ (red, right) and counterstained with DAPI (blue, left). e Confocal images of Rr5 iPSCs immunostained for HP1γ (red) and Nanog (inset, green). Asterisks indicate feeder layer MEF cells in (d) and (e). Scale bars for (ae) = 15 μm. f Time lapse spinning disk confocal images of ESCs expressing the endogenous HP1β fused to mCherry induced to differentiate with 1 μM of retinoic acid (RA) for 40 hours (see also Additional file 7 for a video)

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