Skip to main content
Fig. 4 | Genome Biology

Fig. 4

From: Heterochromatin Protein 1β (HP1β) has distinct functions and distinct nuclear distribution in pluripotent versus differentiated cells

Fig. 4

HP1β has different interacting partners and functions in pluripotent and differentiated cells. a The number of HP1β interacting partners identified by liquid chromatography-tandem mass spectrometry in MEFs, and in ESCs excluding hits found in control samples. Hits identified in both cell types are indicated. b Reprogramming experiments. Alkaline phosphatase (AP) staining of iPSCs induced from WT MEFs and HP1β KO MEFs after 12 days of reprogramming in identical conditions. c Phase contrast images of WT (left), HP1α KO (middle) and HP1β KO (right) iPSC colonies cultured in standard conditions at passage 1 (top) or passage 6 (bottom) after isolation from the reprogramming plate. HP1β KO iPSCs gave rise to flat and spontaneously differentiating cells (red outline), similar to HP1β KO ESCs. Scale bar = 200 μm. d Graphic summary of HP1β localization and function in pluripotent versus differentiated cells. In WT cells, HP1β is highly expressed and diffuse in ESC and iPSC nuclei whereas it decreases in differentiated cells and associates mostly with pericentric heterochromatin. HP1β KO pluripotent cells do not maintain a proper pluripotent state and tend to differentiate spontaneously; differentiating cells lacking HP1β display skewed differentiation, and reprogramming is facilitated in the absence of HP1β

Back to article page